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International Journal ofAyurvedic and Herbal Medicine
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Effects of antioxidants and gelling agents on regeneration, in vitro conservation and genetic stability of Bacopa monnieri (L.) Pennell.

Published:10 Mar 2011
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A. Yusuf1, RajeshKumar T1*, S.Nikhilesh2, P.S.Rao3

1 Biotechnolgy Division, Department of Botany, University of Calicut, Kerala- 673 635,India

2 Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA

3 R&D Centre for Life Sciences, Dayananda Sagar Institutions, Kumaraswamy Layout, Bangalore-560 078, India

Corresponding Author:RajeshKumar T

picture_as_pdfDownload link10.47191/ijahm/v1i3.01

subjectAbstract

Shoot regeneration of Bacopa monnieri was achieved, on Murashige and Skoog (MS) medium supplemented with 4.4 µM benzyl aminopurine (BA) from different explants. Nodal explants initiated 75-80 shoots, leaf explants 55-65 shoots and internodal explants 50-55 shoots within 40 days of culture. The initiated shoots were multiplied in MS medium containing 2.2 μΜ ΒΑ. Ascorbic acid (50 mg/l) and citric acid (25 mg/l) increased the number of shoots and conservation period of the cultures. Isabgol, when used as gelling agent provided consistent multiplication of cultures compared to microbiology grade agar and cultures were conserved in medium containing 3% (w/v) isabgol for a period of 190 days as against an average of 120 days in 1% (w/v) agar containing media. The conservation period was increased to a period of 200 days in medium gelled with 3% (w/v) isabgol containing 50 mg/l ascorbic acid and 25 mg/l citric acid and more than 80% of the cultures survived in this medium. Rooting was achieved ex vitro by culturing the isolated shoots in autoclaved soilrite moistened with 1/10 strength MS salts. Rooted plantlets were established in soil. Genetic stability assessment with random amplified polymorphic DNA (RAPD) primers showed no variation in the banding pattern of the cultures grown in different concentrations of growth regulators, agar or isabgol and additives containing media. These studies provide a feasible in vitro multiplication and conservation method and genetic stability assessment of B. monnieri cultures.

sellKeywords

Bacopa monnieriAscorbic acidCitric acidIsabgolRAPDGenetic stability

format_list_numberedReferences

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How to Cite

Yusuf, A., T, R., S.Nikhilesh, & P.S.Rao (2011). Effects of antioxidants and gelling agents on regeneration, in vitro conservation and genetic stability of Bacopa monnieri (L.) Pennell.. International Journal of Ayurvedic and Herbal Medicine, 1(3). https://doi.org/10.47191/ijahm/v1i3.01

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