Volume 11, Issue 1

In present era, the sedentary lifestyle is giving birth to so many diseases. So, its need of an hour to prevent ourselves from such types of conditions and this can be done by following proper dincharya, ritucharya and other Ayurvedic regimes. Along all these regimes, shadkriyakala plays a very important role to diagnose disease at proper time. Whole pathogenesis is known as kriyakala. It includes six stages that’s why it is known as shadkriyakala. These are the following six stages – Sanchaya, Prakopa, Prasara, Sthanasamshraya, Vyaktavastha, Bhedavastha. By the help of kriyakala, recognition of disease at its early stage can be done and proper treatment can be taken. So, further in this article the description of all these stages has been done.

Present generation is anxious about lifestyle disorders and ways to lead a healthy wellbeing. Modern science emphasis the role of genetic factors in physical appearance, mental characteristicsand disease formation of individuals. Even though physical structure, appearance and behavior of every individual is different, there are some common traits transferred from parents to offspring. This transmission is explained in terms of genetics. Ancient AyurvedicAcharyas understands and explains this in terms of Beeja, beejabhaga and beejabhagavayava.Theformation and development of embryo is given much importance in Ayurvedic science. The concept of genetics is spread all over the samhita especially in shaareerasthaanain a hidden form. Understanding the concept behind each topic is a matter of interest and it put light on the view of genetics.Ayurvedic science not only gives importance to anatomical, physiological and pathological aspects of genetics but also emphasize the measures to prevent it. The genetics in Ayurveda is hence given much importance as the occurrences of genetic disorders are increasing day by day. Hence this study focuses to understand the role of Beeja, Beejabhagaand Beejabhagaavayava in genetic disorders and genetic predisposition.

The present manuscript describes a rapid, novel, simple, precise, accurate, and robust HPLC method development and validation for simultaneous estimation of aloe emodin, rhein and piperine in a polyherbal formulation. The proposed chromatographic method was carried out isocratically, in a short run time of 10 minutes using Prontosil C18 (250 × 4.6 mm) SH 5.0 µm, usingmobile phase acetonitrile and water (0.05% orthophosphoric acid) in the ratio 55:45. Flow rate was set at 1 mL/min, and the UV detection was at 257 nm. Retention time of aloe emodin, rhein and piperinewas found to be 7.064 ± 0.2 min, 7.580 ± 0.2 min and 8.800 ± 0.2 min respectively. Calibration curves were linear over the concentration range of 1 to 30µg/mL.Regression coefficient were 0.998, 0.996, 0.999 for aloe emodin, rhein and piperinerespectively. This and can be used to for routine analysis of aloe emodin, rhein and piperine in polyherbal formulations, thus conforming to the need of ensuring safety and quality of herbal formulations.