Detection of Biomarker: Resveratrol using HPTLC

Cranberry, a natural occurring fruit, has been used for centuries in traditional medicine to promote urinary tract health. Cranberry extracts marketed as nutraceutical supplements and also for managing a variety of ailments, including microbial infections, metabolic disorders, type 2 diabetes and cancer. Resveratrol (3, 4′, 5-trihydroxystilbene) is a naturally occurring phytoalexin found in Cranberry extract. As a phenolic compound, it contributes to the antioxidant potential. Resveratrol has been shown to regulate the metabolism of lipids, and to inhibit the oxidation of low-density lipoproteins and the aggregation of platelets. Furthermore, as phytoestrogen, resveratrol may provide cardiovascular protection. Additionally, this compound possesses anti-inflammatory and anticancer properties. In light of this, the current study involved an attempt to quantify Resveratrol, an active marker, in two commercially available formulations of Cranberry extract. The mobile phase was optimized and Resveratrol bands were visualized on the TLC (Thin Layer Chromatography) plates.