HPTLC, Pharmacognostic standardisation and Biological activity of Cannabis sativa

Cannabis sativa belongs to the family Cannabinaceae and has notable medicinal properties as it is used as for psychoactive effects. Cannabis which contains CBD and THC exhibits a range of potential therapeutic benefits, including anti-inflammatory, analgesic, anxiolytic and neuroprotective properties. Methanolic extracts of Cannabis sativa were obtained through reflux extraction to perform further experiments. Various experimental tests were carried out to understand the physicochemical, fluorescence properties of Cannabis sativa. HPTLC and phytochemical profiles were also obtained. Preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, Tannins, Phenols and steroids. Microscopy of the sample powder showed the presence of starch, stigma shaped papillae and Calcium oxalate crystals. HPTLC profiling showed 9 bands of a pattern unique to Cannabis sativa. The Cannabis sativa showed antidiabetic and anti oxidant activity when bioautography was done.