ISOLATION OF ACTIVE COMPONENTS DERIVED FROM RHIZOME OF EUPHORBIA WALLICHII HOOK
1 Pest Control and Ayurvedic drug Research Lab SSL, Jain P. G. College Vidisha M.P
2 Department of Botany Govt. Degree College Kupwara, J&K, India 193222
3 Department of Botany Govt. Degree College Handwara, J&K, India
Corresponding Author:Firdous Ahmad malla
The objective of the present investigation was to isolate the active components present in rhizome of euphorbia wallechii. The plant material were extracted with various solvents (pet. ether, methanol and water), water was found to be more active among them. Preliminary phytochemical investigation of the aqueous extract of the rhizome of euphorbia wallechii showed that the saponins, steroids, and triterpenoids were found more active among the active constituents. Whereas flavonoids, Cardiac Glycosides showed the less positive nature. The aqueas water extract of Euphorbia wallichii was undergone column chromatography with different solvent fractions. Fraction E which was obtained after purification as a cream color powdered material with the elution of 10 % ethyl acetate in hexane: chloroform and methanol: water from the silica gel column loaded methanol soluble fraction of the plant. It was noticed that melting point apparatus measured 210-212 °C. Hence, it was concluded that fraction E was found very close to the multiflorenol (multiflorane) class based up on the spectral analysis the compound was named as Pentacyclic tri-terpenoid saponin. The structure of the isolated compound was characterized by using IR, CNMR, HNMR and Mass spectrophotometric methods. Therefore, further biological investigations needs to be carried out on isolated compounds present in this plant.
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How to Cite
malla, F. A., Lone, F. A., & Sofi, M. A. (2013). ISOLATION OF ACTIVE COMPONENTS DERIVED FROM RHIZOME OF EUPHORBIA WALLICHII HOOK. International Journal of Ayurvedic and Herbal Medicine, 3(3). https://doi.org/10.47191/ijahm/v3i3.02
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