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International Journal ofAyurvedic and Herbal Medicine
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DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR DETERMINATION OF 6-GINGEROL IN SOFT GELATIN CAPSULE CONTAINING GINGER OLEORESIN

Published:14 Aug 2014
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Keshwar Unmesh1*, Pimplapure Soumit1, Dhurde Ss1, Shrikhande Bk1

1 Baidyanath Life Sciences Pvt. Ltd, Nagpur, India

Corresponding Author:Keshwar Unmesh

picture_as_pdfDownload link10.31142/ijahm/v4i4.03

subjectAbstract

A sensitive and reliable HPTLC method has been developed for quantitative estimation of 6-gingerol in the soft gelatin capsule containing Ginger oleoresin. Chromatography was performed on silica gel 60 F254 percolated TLC plate using n-hexane: ether (4.0: 6.0, v/v) as solvent system and densitometric determination was carried out by TLC scanner (CAMAG) at 254 nm in reflectance/absorbance mode. The Rf value of 6-gingerol was found to be 0.27 ± 0.01. Linearity was found to be in the concentration range of 200 ng to 1400 ng. The linear regression data for the calibration plots showed a good linear relationship with r²=0.997 for 6-gingerol. The accuracy of the method was checked by conducting recovery studies at three different levels, using the standard addition method. The average recovery of 6-gingerol was found to be 99.96%. The proposed HPTLC method provide a good resolution of 6-gingerol and can be used for quantification of 6-gingerol present in soft gelatin capsule. The method is rapid, simple and precise.

sellKeywords

HPTLCgingerolSoft gelatin capsuleGinger oleoresinZingiber officinale

format_list_numberedReferences

  1. Indian Herbal Pharmacopoeia. Revised new Edn, Indian Drug Manufacturers Association, Mumbai, 2002, 479-490.
  2. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Edn 33, Nirali Prakashan, Pune, 2005, 397-398.
  3. Nadkarni KM. Indian Materia Medica. Edn 3, Vol I, Popular Prakashan, Mumbai, 1976, 1308-1315.
  4. Mukherjee PK. Quality Control of Herbal Drugs. Edn 1, Business Horizones, New Delhi, 2002, 767-770.
  5. Handa SS, Kapoor VK. Pharmacognosy. Edn 2, Vallabh Prakashan, Delhi, 1988, 242-244.
  6. Rajpal V. Standardisation of Botnicals. Vol I, Eastern publishers, New Delhi, 2002, 262-270.
  7. Evans WC. Trease and Evans Pharmacognosy. Edn 15, Elsevier publication, 2002, 281.
  8. Kalia AN. Textbook of Industrial Pharmacognosy. Edn 1, CBS publishers, New Delhi, 2005, 228.
  9. Grzanna R, Lindmark L, Frondoza CG. Ginger--an herbal medicinal product with broad anti-inflammatory actions. Journal of Medicinal Food 2005; 8(2):125-32.
  10. Thomson M, Al-Qattan KK, Al-Sawan SM, Alnaqeeb MA, Khan I, Ali M. The use of ginger (Zingiber officinale Rosc.) as a potential anti-inflammatory and antithrombotic agent. Prostaglandins, leukotrienes, and essential fatty acids 2002; 67(6): 475-8.
  11. Nicoll R, Henein MY. Ginger (Zingiber officinale Roscoe): a hot remedy for cardiovascular disease. International Journal of Cardiology 2009; 131(3):408-9.
  12. Ali BH, Blunden G, Tanira MO, Nemmar A. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food and chemical toxicology: An International Journal published for the British Industrial Biological Research Association 2008; 46(2): 409-20.
  13. He X, Bernart MW, Lian L, Lin L. High-performance liquid chromatography-electrospray mass spectrometric analysis of pungent constituents of ginger. Journal of Chromatoghaphy 1998; 796(2): 327-334.
  14. Rai S, Mukherjee K, Mal M, Saha BP, Mukherjee PK. Determination of 6-gingerol in ginger (Zingiber officinale) using high-performance thin-layer chromatography. Journal of Separation Science 2006; 29(15): 2292-2295.
  15. Schwertner HA, Rios DC. High-performance liquid chromatographic analysis of 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol in ginger-containing dietary supplements, spices, teas, and beverage. Journal of Chromatoghaphy 2007; 865(1-2): 41-47.
  16. Smith RM. Analysis of the pungent principles of ginger and grains of paradise by high-performance liquid chromatography using electrochemical detection. Chromatographia 1982; 16: 155-157.
  17. Alam P. Densitometric HPTLC analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams. Asian Pacific Journal of Tropical Biomedicine 2013; 3(8): 634-638.
  18. Mishra AP, Saklani S, and Chandra S. Estimation of gingerol content in different brand samples of ginger powder and their anti-oxidant activity: A comparative study. Recent Research in Science and Technology 2013; 5(1): 54-59.
  19. K. Senthil Kumar KS, Manasa B, Rahman K, Sudhakar B. Development and validation of hptlc method for estimation of 6-gingerol in herbal formulations and extracts. International Journal of Pharmaceutical Science and Research 2012; 3 (10): 3762-3765.
  20. ICH Harmonized Tripartite Guideline. Validation of Analytical Procedures: Text and Methodology. Q2 (R1), November 2005.

How to Cite

Unmesh, K., Soumit, P., Ss, D., & Bk, S. (2014). DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR DETERMINATION OF 6-GINGEROL IN SOFT GELATIN CAPSULE CONTAINING GINGER OLEORESIN. International Journal of Ayurvedic and Herbal Medicine, 4(4). https://doi.org/10.31142/ijahm/v4i4.03

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