Isolation Of Active Components Derived From Whole Plant Of Borreria hispida (Linn.)

The objective of the present investigation was to isolate the active components present in whole plant of Borreria hispida. The plant were extracted with various solvents (pet. ether, ethyl acetate and methanol), methanol was found to be more active among them. The preliminary phytochemical results revealed that flavonoids and amino acids as active constituents in methanolic extract of Borreria hispida. The methanolic extract of Borreria hispida was undergone column chromatography with different solvent fractions. Hence, two compounds were isolated from methanolic extract of Borreria hispida with the compound 1 was eluted with benzene: Chloroform 70:30, v/v and compound 2 were eluted with eluted with ethyl acetate: methanol 50:50, v/v. The structures of the two isolated compounds were characterized by using FT-IR, NMR and Mass spectrophotometric methods. Thus, the compound 1 was characterized as 1-amino-1-ethoxypropan-2-ol (C5H13O2N), and compound 2 was characterized as 3,5,7-trihydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one(C15H12O6). Therefore, further biological investigations need to be carried out isolated compounds present in this plant.