Identification And Determination of Bioactive Polyphenols of V. Vinefera Forphyto-Therapeutic Applications

Vitisvinifera is one of the important economic fruit crop having widespread application in variety of health diseases and disorders. Since anancient times, Vitisvineferaissignificantly used in Ayurvedadue presence of bioactivepolyphenols..Although, itsfruits and seedshas been used in various AyurvedicRasayandrugs and formulations, scanty research has been carried out on presence of polyphenols in aerial parts of this plants and their study asa remedial agent.The present study deals with identification and determination of bioactive polyphenols in Black and White(Green) cultivar of V.vinefera .Organic extracts were prepared by soaking the dry powder into 90% Methanol. Qualitative analysis of different parts of V. vinefera viz. leaf lamina, stem and petiole was carried out by phytochemical screening. In the quantitative analysis, Folin-Ciocalteau method was used to determine total phenolic content whereas, total flavonoid content was estimated by aluminum chloride colorimetric assay. Free radical scavenging activity of extract wascarried out by DPPH assay andaccumulation of bioactive compounds in different regions was studied by anatomical analysis. Results of phytochemical screening of both cultivars showed presence of bioactive polyphenols viz. flavonoids, ellagic acid, tanninalong with otherphenolic compoundsin high abundance.In the white cultivartotal phenolic content of petiole was found to be highest (1.44± 0.19 mg/g GAE) whereas,leaves showed lowest content (0.38± 0.11mg/g GAE).In the black cultivar, leaves showed highest content of total phenols (1.57± 0.05 mg/g GAE) and stem showed lowest content i.e. 0.41± 0.07 mg/g GAE.Total flavonoid content of petiole of white cultivar was observed to behighest (0.15 ±0.05mg/g quercetin equivalent)as compared to leaves and stem.Whereas, leaves of black cultivar showed almost high content of flavonoid i.e. 0.26 ±0.09mg/g quercetin equivalent.Order of radical scavenging activity forboth cultivar was,petiole(79.24%)>stem(75.10%)>leaflamina(72.37%)and stem(83.03%)> petiole(80.40 %)>leaflamina(62.42%) respectively.Amount of flavonoids (quercetin) in petiole of white cultivar was found to be 3.27 ppm by HPLC analysis.Findings of the current study revealed that aerial parts of V. vineferaare rich source of various bioactive polyphenolic compounds with promising therapeutic potential. Therefore irrespective to economic boundaries, these aerial parts can be efficiently usedin for phyto-therapeutic applications.