Preparation and Evaluation of Mangifera Indica Loaded Ethosomal Gel for Anti-Inflammatory Activity in Animal Model

The study was designed to evaluate the ethosomal gel of leaf extract of Mangifera indica, its incorporation in to gel formulations. The prepared gels were assessed for their anti-inflammatory activity against carrageenan induced oedema. Different formulations of ethosomes using lecithin, cholesterol and ethanol were prepared using different doses of Mangifera indica herbal leaf extracts. Carbopol 940 was used to prepare ethosomal gel. The entrapment efficiency of ethosomes was 65.1%-96.54% and the average vesicle size was 920nm. Three formulations (different doses) were selected based on entrapment efficiency and drug release. Prepared gels were then evaluated for physicochemical characteristics and drug content. In vivo anti-inflammatory activity studies were carried out using carrageenan-induced rat paw oedema model. Male Wistar rats weighing about 150-180 gms were divided into 5 groups of six rats (n=6) each. Diclofenac sodium was used as a standard drug (MaKami pharmacy). Three gel formulations (100, 200 and 300 mg) were used as test drugs. The pH of the gel formulations was found to be in the range of 5.4-6.2 and viscosities were between 2250- 2399 centipoises. The drug content of gels ranged between 74.67-82.31%. Among the prepared formulations of Mangifera indica FN2 (Formulation 2, 200 mg) was found to possess significant percentage inhibition (54%) when compared to control at 4 hrs. From the study, it can be concluded that ethosomal gel served as an efficient drug delivery system for herbal extract with potential anti-inflammatory property.