●Call for Papers: Volume 16, Issue 2 — Submit by March-April 2026●Open Access — All articles freely available worldwide●Call for Papers: Volume 16, Issue 2 — Submit by March-April 2026●Open Access — All articles freely available worldwide
Journal Official Logo
International Journal ofAyurvedic and Herbal Medicine
arrow_backBack to Issue
arrow_backPrevious Article
Next Articlearrow_forward

PROTECTIVE EFFICACY OF THE ETHANOLIC EXTRACT OF ROSMARINUS OFFICINALIS (Linn) AGAINST BRADYKININ INDUCED INFLAMMATION IN RATS

Published:07 Apr 2012
visibility0 Viewsdownload0 Downloads
Kathiravan.S1*, Shwetha.V.Kalava1

1 Kongunadu Arts and Science College, Coimbatore, India

Corresponding Author:Kathiravan.S

picture_as_pdfDownload link10.47191/ijahm/v2i2.19

subjectAbstract

The present study investigates the anti-inflammatory activity of ethanolic extracts of Rosmarinus officinalis Linn. The dried leaves of Rosmarinus officinalis were extracted with ethanol using soxhlet apparatus. The effect was observed on bradykinin induced paw edema in female SD rats. Inflammation was induced by subcutaneous injection of freshly prepared 0.1ml bradykinin (1mg/ml) in the right hind paw. The two treatment group rats received 250mg/kg b.wt and 500mg/kg b.wt respectively orally, one hour prior to bradykinin induction. The increase in the paw thickness in the rats after induction with bradykinin was significantly lowered on treatment with the plant extract. The ethanolic extract was found to improve the antioxidant status in the animals in a dose dependent manner. The effect of the extract was compared with the effect of the standard drug indomethacin administration. Histopathological analysis of the hindpaw tissue supports the protective effect of the ethanolic extract of R. officinalis.

sellKeywords

ROSMARINUS OFFICINALIS

format_list_numberedReferences

  1. Adel kadri, Zied Zarai, Ines Ben Chobba, Ahmed Bekir, Neji Gharsallah, Mohammed Damak et al. Chemical constituents and antioxidant properties of Rosmarinus officinalis L. essential oil cultivated from the south-western of Tunisia. Journal of Medicinal Plants Research 9 December, 2011 Vol. 5(29), pp. 6502-6508
  2. Akira Imadaya, Katsutoshi, Terasawa, Hiroyori, Tosa, Meguni, Okamots, Kazuo, Toriizuka. Erythrocyte antioxidant enzymes are reduced in patients with rheumatoid arthritis. J. Rheumatol., 1988; 15: 11.37.
  3. Allen R.C, Stjernholm R.L and Steele R.H. Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochem. Biophys. Res. Commun., 1972; 47: 679–684.
  4. Aziza kamal genana, Haiko hanse, arthur smania junior, simone machado de souza Rosemary(Rosmarinus officinalis)- a study of the composition, antioxidant and antimicrobial activities of extracts obtained with supercritical carbondioxide Ciênc. Tecnol. Aliment., Campinas, 2008; 28(2): 463-469.
  5. Babior, B.M., Kipens, R.S. and Curnutte, J.T. The production by leukocytes of superoxide dismutase, a potential bactericidal agent. J. Clin. Invest., 1973; 52: 741–744.
  6. Bai S.K, Lee S.J, Na H.J, Ha K.S, Han J.A, Lee H.S, Kwon Y.G, Chung C.K and Kim Y.M. Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulated macrophages by suppressing redox-based NF-B activation. Experimental and Molecular Medicine., 2005; 37: 23–334.
  7. Bourke E and Moynagh P.N. Antiinflammatory effects of glucocorticoids in brain cells independent of NF-kappa B. Journal of Immunology, 1999; 163: 2113–2119.
  8. Busnardo TC, Padoani C, Mora TC, Biavatti MW, Fröde TS, Bürger C et al. Anti-inflammatory evaluation of Coronopus didymus in the pleurisy and paw oedema models in mice. J Ethnopharmacol. 2010 Mar 24; 128(2):519-25.
  9. D'Acquisto F., May M.J and Ghosh S. Inhibition of nuclear factor kappa B (NF-B): an emerging theme in anti-inflammatory therapies. Molecular Interventions., 2002; 2: 22–35.
  10. DiRosa M, Giroud J.P. and Willoughby D.A. Studies on the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J. Pathol. 1971; 104: 15-29.
  11. Elhoussine derwich, Zineb benziane, Rachida chabir and Rachid taouil. Invitro antibacterial activity and GC/MS analysis of the essential oil extract of leaves of rosmarinus officinalis grown in Morocco. Int J Pharm Pharm Sci, 2011; 3(3):89
  12. Elhoussine derwich, Zineb benziane, Rachida chabir, Rachid taouil. Invitro antibacterial activity and GC/MS analysis of the essential oil extract of leaves of Rosmarinus officinalis grown in morocco. Int J Pharm Pharm Sci, 2011:Vol 3, Issue 3, 89-95.
  13. Forman, H.J. and Torres, M. Redox signaling in macrophages. Molecular Aspects of Medicine., 2001; 22: 189-216.
  14. Frei B, England L and Ames B. Proc. Natl. Acad. Sci. USA, 1989; 86: 6377–6381.
  15. Fridovich I. Superoxide dismutase. Annu Rev Biochem., 1974; 44:147–159.
  16. Ginn-Pease M.E and Whisler R.L. Redox signals and NF-_B activation in T cells. Free Radical Biology and Medicine., 1998; 25: 346–361.
  17. Green H.C.C, Wagner D.A and Glogowski J. Analysis of nitrate, nitrite and (¹5N) nitrate in biological fluids. Anal. Biochem 1982;126: 131-138.
  18. Gulcin I, Sat I. G, Beydemir S, Elmastas M and Kufreviog lu O I. Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb) buds and lavender (Lavandula stoechas L.) Food Chem, 2004; 87: 393–400.
  19. Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT and Hartzfeld PW. High molecular weight plant polyphenolics(tannins) as biological antioxidants. J Agric and Food Chem. 1998; 46: 1887-1892.
  20. Haraguchi H, Saitao T and Ishikawa H. Antiperoxidative components in thymus vulgaris. Planta Med 1996; 62: 217-221.
  21. Hogg N. Free radicals in disease. Seminar in Reproductive Endocrinology, 1998; 16: 241–248.
  22. Kasapoglu M. and Ozben T. Alterations of antioxidant enzymes and oxidative stress markers in aging. Exp. Gerontol., 2001; 36: 209– 220.
  23. Kawamura T, Ohisa Y, Abe Y, Ishimori A, Shineha R. and Yokota, K. Plasma lipid peroxides in the operation of oesophageal cancer. Rinsho-Ayori, 1992; 40: 881–884 (in Japanese).
  24. Keizo kohno, Masaki miyake, Osamu sano, Mari tanaka-kataoka, Shigeto yamamoto, Satomi koya-miyata et al. Anti-inflammatory and Immunomodulatory Properties of 2-amino-3H-phenoxazin-3-one.Biol.Pharm.Bull. 2008;31(10): 1938
  25. Lamaison J.L.C and Carnet A. Teneurs en principaux flavonoids des fleurs de Crataegeus monogyna Jacq et de Crataegeus laevigata (Poiret D. C) en function de la vegetation. Pharm. Acta. Helv., 1990;65: 315-320.
  26. Lefkowitz D.L, Gelderman M.P and fuhrmann S.R. Neutrophilic lysozyme-macrophage interactions perpetuate chronic inflammation associated with experimental arthritis. Clin Immunol 1999; 91: 145-155.
  27. Lowry O.H, Roseobrough N.J, Farr A.L and Randall R.J. Protein measurement with the Folin's phenol reagent. J. Biol. Chem. 1957; 193: 265-275.
  28. Mahat M.A and Patil B.M. Evaluation of anti-inflammatory activity of methanol extract of Phyllanthus amarus in experimental animal models. Indian J. Phar Sci. Jan Feb. 2007; 33-36.
  29. Mattson M.P, Duan W, Pedersen W.A, Culmsee C. Neurodegenerative disorders and ischemic brain diseases. Apoptosis 2001; 6: 69–81.
  30. Mitchell R.N, Cotran R.S. In:Robinsons Basic Pathology, Harcourt Pvt. Ltd; New Delhi, India, 2000; ed.7;33-42.
  31. Moron M.S, Defierre J.W and Mannervik B.. Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochem. Biophys. Acta. 1979; 582: 67-68.
  32. Nakamura Y, Tsuji S and Tonogai Y. Analysis of proanthocyanidins in grape seed extracts, health foods and grape seed oils. J.Health.Sci. 2003; 49:45-54.
  33. Nguyen Thi Dung, Vivek K.Bajpai, Jung In Yoon, Sun Chul Kang. Anti-inflammatory effects of essential oil isolated from the buds of Cleistocalyx operculatus(Roxb.) Merr and Perry. Food and chemical toxicology 2009; 47: 449-453.
  34. Niehius W.G. and Samuelsson D. Formation of malondialdehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur. J. Biochem. 1968;6: 126-130.
  35. Omabola oluranti okoh. Chemical transformations and phytochemical studies of bioactive components from extracts of Rosmarinus officinalis L.2010; 35.
  36. Omaye S.T, Turnball T.D and Sauberlich H.E. Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Methods. Enzymol. 1979; 62: 1-11.
  37. Pincus, T., Marcum, S.B., Callahan, L.F. Long-term drug therapy for rheumatoid arthritis in seven rheumatology private practices. II. Second line drugs and prednisone. The Journal of Rheumatology 1992; 19: 1885-1894.
  38. Rice-Evans C.A, Miller J and Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci., 1997; 2: 152–159.
  39. Rice-Evans C.A, Miller N.J and Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med1996; 20: 933–956.
  40. Robert E.B. Method for estimation of tannin in grain sorghum. Agro. J., 1971; 63: 511.
  41. Salin M.L, McCord J.M. Free radicals and inflammation: protection of phagocytosing leucocyte by SOD. J. Clin. invest. 1975; 56: 1319.
  42. Scott D.L, Shipley M, Dawson A, Edwards S, Symmons D.P, Woolf A.D. The clinical management of rheumatoid arthritis and osteoarthritis: strategies for improving clinical effectiveness. British Journal of Rheumatology 1998; 37:546–554.
  43. Sies H. Biochemistry of oxidative stress. Angewandt Chemie International Edition in English, 1991; 25: 1058-1071.
  44. Singleton V.L and Rossi J.A. Colorimetry of total phenolics with phosphotungstic acid reagents. Am. J. Enol. Viticul. 1965; 16: 144-158.
  45. Sinha A. K. Colorimetric assay of catalase. Anal. Biochem1972; 47: 389-394
  46. Sumanth,M and Rana,A.C.. Invitro antioxidant activity of hydroalcoholic extracts of Taxarum officinale roots in rats Indian J Pharmacol., 2006; 38(1): 54-5.
  47. Tillet W.S, and Francis T, J.Exter, Med, 1930; 52: 561.
  48. Valentao P, Fernandes E, Canvalho E, Andrade P.B, Seabra R.M and Bastos M.L. Antioxidant activity of Hypericum androsaenium infusion scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. Biol Pharm Bull 2002; 25: 1324-1327.
  49. Veiga JF, Zunino, Patitucci MP, Pinto AC and Calixto JB. The inhibition of paw oedema formation caused by the oil of Copaifera multijuga hane and its fractions. Pharm. Pharmcol. 2006; 2042-2158
  50. Yoke Keong Y, Arifah AK, Sukardi S, Roslida AH, Somchit MN, Zuraini A. Bixa orellana leaves extract inhibits bradykinin-induced inflammation through suppression of nitric oxide production. Med Princ Pract. Epub 2011 Jan 20.20(2):142-6.
  51. Yoshikawa T, Naito Y. The role of neutrophils and inflammation in gastric mucosal injury. Free Radic Res 2000; 33: 785–794.

How to Cite

Kathiravan.S & Shwetha.V.Kalava (2012). PROTECTIVE EFFICACY OF THE ETHANOLIC EXTRACT OF ROSMARINUS OFFICINALIS (Linn) AGAINST BRADYKININ INDUCED INFLAMMATION IN RATS. International Journal of Ayurvedic and Herbal Medicine, 2(2). https://doi.org/10.47191/ijahm/v2i2.19

APA

American Psychological Association Style

arrow_back
Previous ArticleAnxiolytic Activity Of Camellia Sinensis Extract
Next ArticleImportance Of Bhaisajya Kala In The Management Of Diseases
arrow_forward